Amino acid analysis
نویسندگان
چکیده
منابع مشابه
Amino acid analysis.
Amino acid analysis (AAA) is one of the best methods to quantify peptides and proteins. Two general approaches to quantitative AAA exist, namely, classical postcolumn derivatization following ion-exchange chromatography and precolumn derivatization followed by reversed-phase HPLC (RP-HPLC). Excellent instrumentation and several specific methodologies are available for both approaches, and both ...
متن کاملAdvances in Amino Acid Analysis
amino acid profiling in routine clinical analysis (1,2). As analysts started using capillary electrophoresis (CE) in the 1990s, they were able to successfully use most of the popular reagents from LC analyses in electromigration techniques. The remarkable progress in detection technologies — such as diodearray ultraviolet (UV), laser-induced fluorescence, evaporative light-scattering, and mass ...
متن کاملBayesian analysis of amino acid substitution models.
Models of amino acid substitution present challenges beyond those often faced with the analysis of DNA sequences. The alignments of amino acid sequences are often small, whereas the number of parameters to be estimated is potentially large when compared with the number of free parameters for nucleotide substitution models. Most approaches to the analysis of amino acid alignments have focused on...
متن کاملTotal fluorometric amino acid analysis using fluorescamine.
A fllwrometric amino acid analyzer using fllmresramine for the assay of the full array of natural amino ac<ids including prolinc on a single column is reported. The proline determination was carried out by specific introduction of a solution of ,\‘chlorosl~ccinimide into the flow system. Single rolumn fluorometric amino acid analysis was curried out in a significantly shorter time and with a se...
متن کاملá1052ñ BIOTECHNOLOGY-DERIVED ARTICLES—AMINO ACID ANALYSIS
This chapter provides guidance and procedures used for characterization of biotechnology-derived articles by amino acid analysis. This chapter is harmonized with the corresponding chapter in JP and EP. Portions of the chapter that are not harmonized with the other two pharmacopeias are marked by the symbol ♦. The footnote below is in the USP but is not in the EP or JP. Other characterization te...
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ژورنال
عنوان ژورنال: Nature
سال: 1979
ISSN: 0028-0836,1476-4687
DOI: 10.1038/279173a0